Chitosan Nanoparticles for Gene Delivery in Macrobrachium Rosenbergii (de Man 1879)
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چکیده
The present study demonstrates non viral gene delivery using chitosan nanoparticles in Macrobrachium rosenbergii as model organism. Chitosan nanoparticles were synthesized by ionic gelation method. The mean particle size of chitosan nanoparticles was 27.6nm. The Polydispersity index was found to be 0.448 and zeta potential was +24.79mV. Green fluorescent protein encoding plasmid (PDB-402) was used for conjugation with these nanoparticles. Chitosan (nanoparticle concentration: 40mg/L) DNA nanoconjugates (plasmid concentration: 50ng/μl) were administered to prawns via immersion treatment for a period of 36 hrs. The gene expression was studied using PCR and fluorescence microscopy which showed green fluorescent protein expression in muscle, gills and pleopods indicating wide tissue distribution. This study also affirms that immersion treatment can be efficiently employed for DNAnanoconjugate delivery as it minimises the stress in animals.
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